ELK050ES-48T, EasyStep Rat fT3(Free Triiodothyronine) ELISA Kit, 48T

Alternative Names: 3,3',5-Triiodo-L-Thyronine; Liothyronine; Cytomel; Tertroxin

Species: Rat

Assay Type: Competitive Inhibition

Sensitivity: 0.31 ng/mL

Standard: 20 ng/mL

Detection range: 0.32-20 ng/mL

Sample type: Serum, plasma

Assay length: 1.5h

Research Area: Endocrinology;Autoimmunity;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat fT3 antigen, and the Rat fT3 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Rat fT3 to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Rat fT3 in the samples is then determined by comparing the OD of the samples to the standard curve.