ELK0506-96T, Human Anti-GAD65(Anti-Glutamate Decarboxylase 65 Autoantibodies) ELISA Kit, 96T

Alternative Names: anti-GAD65; anti-Pancreatic Islets And Brain,65kDa; anti-65 kDa glutamic acid decarboxylase; anti-Glutamate decarboxylase 65 kDa isoform

Species: Human

Assay Type: Sandwich

Sensitivity: 1.31 IU/mL

Standard: 250 IU/mL

Detection range: 3.91-250 IU/mL

Sample type: tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 3.5h

Research Area: Enzyme & Kinase;Endocrinology;Neuro science;Autoimmunity;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-GAD65. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-GAD65. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-GAD65, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-GAD65 in the samples is then determined by comparing the OD of the samples to the standard curve.