ELK0502-48T, Mouse BPA(Bisphenol A) ELISA Kit, 48T

ELK0502-48T, Mouse BPA(Bisphenol A) ELISA Kit, 48T

ELK0503-48T, Human ZKSCAN1(Zinc finger with KRAB and SCAN domains 1) ELISA Kit, 48T

ELK0503-48T, Human ZKSCAN1(Zinc finger with KRAB and SCAN domains 1) ELISA Kit, 48T

ELK0502-96T, Mouse BPA(Bisphenol A) ELISA Kit, 96T

2.963,10 RON

Mouse BPA(Bisphenol A) ELISA Kit

SKU
ELK0502-96T

Alternative Names: BPA

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.68 ng/mL

Standard: 100 ng/mL

Detection range: 1.57-100 ng/mL

Sample type: Plasma, Serum

Assay length: 3.5h

Research Area: Neuro science;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse BPA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse BPA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse BPA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse BPA in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: BPA

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.68 ng/mL

Standard: 100 ng/mL

Detection range: 1.57-100 ng/mL

Sample type: Plasma, Serum

Assay length: 3.5h

Research Area: Neuro science;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse BPA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse BPA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse BPA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse BPA in the samples is then determined by comparing the OD of the samples to the standard curve.