ELK0497-48T, PD1(Protectin D1) ELISA Kit, 48T

Alternative Names: Neuroprotectin D1; NPD1

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 0.072 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: Serum, plasma and other biological fluids.

Assay length: 2.5h

Research Area: Immunology & Inflammation

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with PD1 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PD1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PD1 in the samples is then determined by comparing the OD of the samples to the standard curve.