ELK0458-96T, Human FATP6(Fatty Acid Transport Protein 6) ELISA Kit, 96T

Alternative Names: SLC27A6; ACSVL2; FACVL2; FATP6; Fatty-acid-coenzyme A ligase, very long-chain 2; Solute carrier family 27 member 6; Very long-chain acyl-CoA synthetase homolog 1

Species: Human

Assay Type: Sandwich

Sensitivity: 0.071 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: Tissue homogenates and other biological fluids.

Assay length: 3.5h

Research Area: Metabolic pathway;Cardiovascular biology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FATP6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FATP6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FATP6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FATP6 in the samples is then determined by comparing the OD of the samples to the standard curve.