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ELK0451-48T, Mouse T4(Thyroxine) ELISA Kit, 48T
1.814,75 RON
Mouse T4(Thyroxine) ELISA Kit
SKU
ELK0451-48T
Alternative Names: 3,5,3',5'-Tetraiodothyronine; L-Thyroxine; Levothyroxine
Species: Mouse
Assay Type: Competitive Inhibition
Sensitivity: 1.49 ng/mL
Standard: 300 ng/mL
Detection range: 4.69-300 ng/mL
Sample type: serum, plasma and other biological fluids
Assay length: 2.5h
Research Area: Endocrinology;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse T4 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse T4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse T4 in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 1.525,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: 3,5,3',5'-Tetraiodothyronine; L-Thyroxine; Levothyroxine Species: Mouse Assay Type: Competitive Inhibition Sensitivity: 1.49 ng/mL Standard: 300 ng/mL Detection range: 4.69-300 ng/mL Sample type: serum, plasma and other biological fluids Assay length: 2.5h Research Area: Endocrinology;Hormone metabolism; Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse T4 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse T4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse T4 in the samples is then determined by comparing the OD of the samples to the standard curve. |
