ELK042ES-96T, EasyStep Human SAA(Serum Amyloid A) ELISA Kit, 96T

Alternative Names: SA-A; SAA1; PIG4; TP53I4; Amyloid protein A; Amyloid fibril protein AA; Serum amyloid A-1 protein

Species: Human

Assay Type: Sandwich

Sensitivity: 0.15 ng/mL

Standard: 50 ng/mL

Detection range: 0.78-50 ng/mL

Sample type: Serum, plasma

Assay length: 1.5h

Research Area: Infection immunity;Kidney biomarker;Hepatology;Urology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SAA, and the Human SAA standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human SAA. After TMB substrate solution is added, only those wells that contain Human SAA and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SAA in the samples is then determined by comparing the OD of the samples to the standard curve.