ELK038ES-48T, EasyStep Human NGAL(Neutrophil Gelatinase Associated Lipocalin) ELISA Kit, 48T

Alternative Names: LCN2; p25; Lipocalin 2; Oncogene 24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; Siderocalin LCN2

Species: Human

Assay Type: Sandwich

Sensitivity: 5.6 pg/mL

Standard: 1000 pg/mL

Detection range: 15.63-1000 pg/mL

Sample type: Serum, plasma

Assay length: 1.5h

Research Area: Infection immunity;Urology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NGAL, and the Human NGAL standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human NGAL. After TMB substrate solution is added, only those wells that contain Human NGAL and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NGAL in the samples is then determined by comparing the OD of the samples to the standard curve.