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ELK0384-48T, Horse 5-HT(5-Hydroxytryptamine) ELISA Kit, 48T
1.814,75 RON
Horse 5-HT(5-Hydroxytryptamine) ELISA Kit
SKU
ELK0384-48T
Alternative Names: 5HT; Serotonin; N-Methyl-Gamma
Species: Horse
Assay Type: Competitive Inhibition
Sensitivity: 6.12 ng/mL
Standard: 900 ng/mL
Detection range: 14.07-900 ng/mL
Sample type: serum, plasma, tissue homogenates
Assay length: 2.5h
Research Area: Signal transduction;Infection immunity;Neuro science;Gastroenterology;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Horse 5-HT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse 5-HT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse 5-HT in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 1.525,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: 5HT; Serotonin; N-Methyl-Gamma Species: Horse Assay Type: Competitive Inhibition Sensitivity: 6.12 ng/mL Standard: 900 ng/mL Detection range: 14.07-900 ng/mL Sample type: serum, plasma, tissue homogenates Assay length: 2.5h Research Area: Signal transduction;Infection immunity;Neuro science;Gastroenterology; Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Horse 5-HT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse 5-HT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse 5-HT in the samples is then determined by comparing the OD of the samples to the standard curve. |
