ELK0372-48T, Human ERK1/2(Extracellular Signal Regulated Kinase 1/2) ELISA Kit, 48T

Alternative Names: ERK; ERK-2; ERK2; ERT1; MAPK2; NS13; P42MAPK; PRKM1; PRKM2; p38; p40; p41; p41mapk; p42-MAPK

Species: Human

Assay Type: Sandwich

Sensitivity: 0.12 ng/mL

Standard: 20 ng/mL

Detection range: 0.32-20 ng/mL

Sample type: tissue homogenates, cell lysates and other biological fluids

Assay length: 3.5h

Research Area: Signal transduction;Enzyme & Kinase;Apoptosis;Tumor immunity;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ERK1/2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ERK1/2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ERK1/2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ERK1/2 in the samples is then determined by comparing the OD of the samples to the standard curve.