ELK0364-48T, Human TINAGL1(Tubulointerstitial Nephritis Antigen-Like 1) ELISA Kit, 48T

Alternative Names: Glucocorticoid-inducible protein 5; Oxidized LDL-responsive gene 2 protein; OLRG-2; Tubulointerstitial nephritis antigen-related protein; TIN Ag-related protein; TIN-Ag-RP; TINAL; GIS5; LCN7; OLRG2; TINAGL

Species: Human

Assay Type: Sandwich

Sensitivity: 12.5 pg/mL

Standard: 2000 pg/mL

Detection range: 31.25-2000 pg/mL

Sample type: Serum, plasma

Assay length: 3.5h

Research Area: Cytokine;Infection immunity

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TINAGL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TINAGL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TINAGL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TINAGL1 in the samples is then determined by comparing the OD of the samples to the standard curve.