ELK033ES-48T, EasyStep Human VEGFA(Vascular Endothelial Growth Factor A) ELISA Kit, 48T

Alternative Names: VEGF; VEGF-A; VPF; Vascular Endothelial Cell Growth Factor A; Vascular Permeability Factor

Species: Human

Assay Type: Sandwich

Sensitivity: 31 pg/mL

Standard: 5000 pg/mL

Detection range: 78.2-5000 pg/mL

Sample type: Serum, plasma

Assay length: 1.5h

Research Area: Cytokine;Apoptosis;Tumor immunity;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human VEGFA, and the Human VEGFA standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human VEGFA. After TMB substrate solution is added, only those wells that contain Human VEGFA and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VEGFA in the samples is then determined by comparing the OD of the samples to the standard curve.