ELK032ES-48T, EasyStep Human MIg(Monokine Induced By Interferon Gamma) ELISA Kit, 48T

Alternative Names: CXCL9; CMK; Humig; MI-G; SCYB9; Crg-10; Chemokine (C-X-C motif) ligand 9; Small-inducible cytokine B9; Gamma-interferon-induced monokine

Species: Human

Assay Type: Sandwich

Sensitivity: 15.7 pg/mL

Standard: 5000 pg/mL

Detection range: 78.2-5000 pg/mL

Sample type: Serum, plasma

Assay length: 1.5h

Research Area: Cytokine;Infection immunity;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MIg, and the Human MIg standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human MIg. After TMB substrate solution is added, only those wells that contain Human MIg and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MIg in the samples is then determined by comparing the OD of the samples to the standard curve.