ELK030ES-48T, EasyStep Rat ADP(Adiponectin) ELISA Kit, 48T

Alternative Names: GBP28; ApM1; AdipoQ; Acrp30; ACDC; APM1; C1Q And Collagen Domain Containing; Adipocyte Complement-Related Protein Of 30 KDa; Adipose Most Abundant Gene Transcript 1

Species: Rat

Assay Type: Sandwich

Sensitivity: 4.75 ng/mL

Standard: 500 ng/mL

Detection range: 7.82-500 ng/mL

Sample type: Serum, plasma

Assay length: 1.5h

Research Area: Metabolic pathway;Infection immunity;Cardiovascular biology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ADP, and the Rat ADP standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Rat ADP. After TMB substrate solution is added, only those wells that contain Rat ADP and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ADP in the samples is then determined by comparing the OD of the samples to the standard curve.