ELK0302-96T, Pig CKMB(Creatine Kinase MB Isoenzyme) ELISA Kit, 96T

ELK0302-96T, Pig CKMB(Creatine Kinase MB Isoenzyme) ELISA Kit, 96T

ELK0303-96T, Rat PGRN(Progranulin) ELISA Kit, 96T

ELK0303-96T, Rat PGRN(Progranulin) ELISA Kit, 96T

ELK0303-48T, Rat PGRN(Progranulin) ELISA Kit, 48T

2.689,40 RON

Rat PGRN(Progranulin) ELISA Kit

SKU
ELK0303-48T

Alternative Names: PGRN; Acrogranin; GEP; GP88; GRN; PCDGF; PEPI; Proepithelin

Species: Rat

Assay Type: Sandwich

Sensitivity: 21.2 pg/mL

Standard: 4000 pg/mL

Detection range: 62.5-4000 pg/mL

Sample type: Serum, plasma and other biological fluids.

Assay length: 3.5h

Research Area: Cytokine

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PGRN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PGRN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PGRN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PGRN in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: PGRN; Acrogranin; GEP; GP88; GRN; PCDGF; PEPI; Proepithelin

Species: Rat

Assay Type: Sandwich

Sensitivity: 21.2 pg/mL

Standard: 4000 pg/mL

Detection range: 62.5-4000 pg/mL

Sample type: Serum, plasma and other biological fluids.

Assay length: 3.5h

Research Area: Cytokine

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PGRN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PGRN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PGRN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PGRN in the samples is then determined by comparing the OD of the samples to the standard curve.