ELK0281-48T, Human EIF4E2(Eukaryotic Translation Initiation Factor 4E Type 2) ELISA Kit, 48T

Alternative Names: eIF-4E type 2; eIF4E type 2; Eukaryotic translation initiation factor 4E homologous protein; Eukaryotic translation initiation factor 4E-like 3; eIF4E-like protein 4E-LP; mRNA cap-binding protein 4EHP; h4EHP; mRNA cap-binding protein type 3

Species: Human

Assay Type: Sandwich

Sensitivity: 12.1 pg/mL

Standard: 2000 pg/mL

Detection range: 31.25-2000 pg/mL

Sample type: serum, plasma and other biological fluids

Assay length: 3.5h

Research Area: Breast Cancer

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EIF4E2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EIF4E2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human EIF4E2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EIF4E2 in the samples is then determined by comparing the OD of the samples to the standard curve.