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ELK0276-48T, Mouse APOB100(Apolipoprotein B100) ELISA Kit, 48T
1.814,75 RON
Mouse APOB100(Apolipoprotein B100) ELISA Kit
SKU
ELK0276-48T
Alternative Names: Apo-B100
Species: Mouse
Assay Type: Sandwich
Sensitivity: 32 ng/mL
Standard: 5000 ng/mL
Detection range: 78.13-5000 ng/mL
Sample type: Serum, plasma and other biological fluids
Assay length: 3.5h
Research Area: Metabolic pathway;Endocrinology;Cardiovascular biology;Hepatology;Nutrition metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse APOB100. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse APOB100. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse APOB100, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse APOB100 in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 1.525,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: Apo-B100 Species: Mouse Assay Type: Sandwich Sensitivity: 32 ng/mL Standard: 5000 ng/mL Detection range: 78.13-5000 ng/mL Sample type: Serum, plasma and other biological fluids Assay length: 3.5h Research Area: Metabolic pathway;Endocrinology;Cardiovascular biology;Hepatology;Nutrition metabolism; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse APOB100. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse APOB100. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse APOB100, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse APOB100 in the samples is then determined by comparing the OD of the samples to the standard curve. |
