ELK0251-48T, Rat 3-MH(3-Methylhistidine) ELISA Kit, 48T

ELK0251-48T, Rat 3-MH(3-Methylhistidine) ELISA Kit, 48T

ELK0252-48T, Rat ANGPTL1(Angiopoietin Like Protein 1) ELISA Kit, 48T

ELK0252-48T, Rat ANGPTL1(Angiopoietin Like Protein 1) ELISA Kit, 48T

ELK0251-96T, Rat 3-MH(3-Methylhistidine) ELISA Kit, 96T

2.963,10 RON

Rat 3-MH(3-Methylhistidine) ELISA Kit

SKU
ELK0251-96T

Alternative Names: 3MH

Species: Rat

Assay Type: Competitive Inhibition

Sensitivity: 2.61 nmol/mL

Standard: 400 nmol/mL

Detection range: 6.25-400 nmol/mL

Sample type: serum, plasma, tissue homogenates and other biological fluids

Assay length: 3.5h

Research Area: Metabolism

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat 3-MH protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat 3-MH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat 3-MH in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: 3MH

Species: Rat

Assay Type: Competitive Inhibition

Sensitivity: 2.61 nmol/mL

Standard: 400 nmol/mL

Detection range: 6.25-400 nmol/mL

Sample type: serum, plasma, tissue homogenates and other biological fluids

Assay length: 3.5h

Research Area: Metabolism

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat 3-MH protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat 3-MH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat 3-MH in the samples is then determined by comparing the OD of the samples to the standard curve.