ELK0237-96T, Human PSG6(Pregnancy Specific Beta-1-Glycoprotein 6) ELISA Kit, 96T

Alternative Names: PS-beta-G-6; PSBG-6; Pregnancy-specific glycoprotein 6; Pregnancy-specific beta-1-glycoprotein 10; PS-beta-G-10; PSBG-10; Pregnancy-specific glycoprotein 10; Pregnancy-specific beta-1-glycoprotein 12; PS-beta-G-12; PSBG-12; Pregnancy-specific glycoprotein 12

Species: Human

Assay Type: Sandwich

Sensitivity: 0.069 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: serum, plasma, tissue homogenates, cell lysates, breast milk, cell culture supernates and other biological fluids

Assay length: 3.5h

Research Area: Reproductive science;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PSG6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PSG6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PSG6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PSG6 in the samples is then determined by comparing the OD of the samples to the standard curve.