ELK0207-96T, Human AKR1C3(Aldo-keto Reductase Family 1 Member C3) ELISA Kit, 96T

Alternative Names: AK1C3; 17-beta-hydroxysteroid dehydrogenase type 5; 17-beta-HSD 5; 3-alpha-HSD type II, brain; 3-alpha-hydroxysteroid dehydrogenase type 2; 3-alpha-HSD type 2; Chlordecone reductase homolog HAKRb; Dihydrodiol dehydrogenase 3; DD-3; DD3; Dihydrodiol dehydrogenase type I; HA1753; Prostaglandin F synthase; PGFS; Testosterone 17-beta-dehydrogenase 5; DDH1; HSD17B5; KIAA0119

Species: Human

Assay Type: Sandwich

Sensitivity: 0.068 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 3.5h

Research Area: Enzyme & Kinase;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human AKR1C3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AKR1C3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human AKR1C3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AKR1C3 in the samples is then determined by comparing the OD of the samples to the standard curve.