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ELK0198-96T, Rat IgG2a(Immunoglobulin G2a) ELISA Kit, 96T
2.963,10 RON
Rat IgG2a(Immunoglobulin G2a) ELISA Kit
SKU
ELK0198-96T
Alternative Names: IgG2a
Species: Rat
Assay Type: Sandwich
Sensitivity: 2.31 ng/mL
Standard: 400 ng/mL
Detection range: 6.25-400 ng/mL
Sample type: Serum, plasma and other biological fluids.
Assay length: 3.5h
Research Area: Infection immunity;Immune molecule;Hematology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IgG2a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IgG2a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IgG2a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IgG2a in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: IgG2a Species: Rat Assay Type: Sandwich Sensitivity: 2.31 ng/mL Standard: 400 ng/mL Detection range: 6.25-400 ng/mL Sample type: Serum, plasma and other biological fluids. Assay length: 3.5h Research Area: Infection immunity;Immune molecule;Hematology; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IgG2a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IgG2a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IgG2a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IgG2a in the samples is then determined by comparing the OD of the samples to the standard curve. |
