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ELK0185-96T, Mouse HSF1(Heat Shock Transcription Factor 1) ELISA Kit, 96T
2.963,10 RON
Mouse HSF1(Heat Shock Transcription Factor 1) ELISA Kit
SKU
ELK0185-96T
Alternative Names: HSTF1; Heat shock factor protein 1
Species: Mouse
Assay Type: Sandwich
Sensitivity: 0.138 ng/mL
Standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: serum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay length: 3.5h
Research Area: Signal transduction;Tumor immunity;Developmental science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse HSF1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse HSF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse HSF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse HSF1 in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: HSTF1; Heat shock factor protein 1 Species: Mouse Assay Type: Sandwich Sensitivity: 0.138 ng/mL Standard: 20 ng/mL Detection range: 0.32-20 ng/mL Sample type: serum, plasma, tissue homogenates, cell lysates and other biological fluids Assay length: 3.5h Research Area: Signal transduction;Tumor immunity;Developmental science; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse HSF1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse HSF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse HSF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse HSF1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
