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ELK0182-96T, Cattle Testo(Testosterone) ELISA Kit, 96T
2.475,20 RON
Cattle Testo(Testosterone) ELISA Kit
SKU
ELK0182-96T
Alternative Names: T; Testesteron; Testeronun
Species: Cattle
Assay Type: Competitive Inhibition
Sensitivity: 0.10 ng/mL
Standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: serum, plasma, other biological fluids
Assay length: 2.5h
Research Area: Endocrinology;Reproductive science;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cattle Testo protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle Testo. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle Testo in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.080,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: T; Testesteron; Testeronun Species: Cattle Assay Type: Competitive Inhibition Sensitivity: 0.10 ng/mL Standard: 20 ng/mL Detection range: 0.32-20 ng/mL Sample type: serum, plasma, other biological fluids Assay length: 2.5h Research Area: Endocrinology;Reproductive science;Hormone metabolism; Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cattle Testo protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle Testo. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle Testo in the samples is then determined by comparing the OD of the samples to the standard curve. |
