ELK0178-96T, Rat KL-6(Krebs Von den Lungen-6) ELISA Kit, 96T

Alternative Names: KL-6

Species: Rat

Assay Type: Sandwich

Sensitivity: 9.12 U/mL

Standard: 1500 U/mL

Detection range: 23.44-1500 U/mL

Sample type: serum, plasma, tissue homogenates and other biological fluids

Assay length: 3.5h

Research Area: Interstitial lung disease

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat KL-6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat KL-6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat KL-6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat KL-6 in the samples is then determined by comparing the OD of the samples to the standard curve.