ELK0172-48T, Mouse INHbB(Inhibin Beta B) ELISA Kit, 48T

ELK0172-48T, Mouse INHbB(Inhibin Beta B) ELISA Kit, 48T

ELK0173-48T, Human INHbA(Inhibin Beta A) ELISA Kit, 48T

ELK0173-48T, Human INHbA(Inhibin Beta A) ELISA Kit, 48T

ELK0172-96T, Mouse INHbB(Inhibin Beta B) ELISA Kit, 96T

2.963,10 RON

Mouse INHbB(Inhibin Beta B) ELISA Kit

SKU
ELK0172-96T

Alternative Names: INH-BB; Activin AB Beta Polypeptide

Species: Mouse

Assay Type: Sandwich

Sensitivity: 4.88 pg/mL

Standard: 1000 pg/mL

Detection range: 15.63-1000 pg/mL

Sample type: Serum, plasma and other biological fluids

Assay length: 3.5h

Research Area: Tumor immunity;Endocrinology;Reproductive science;Developmental science;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse INHbB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse INHbB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse INHbB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse INHbB in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: INH-BB; Activin AB Beta Polypeptide

Species: Mouse

Assay Type: Sandwich

Sensitivity: 4.88 pg/mL

Standard: 1000 pg/mL

Detection range: 15.63-1000 pg/mL

Sample type: Serum, plasma and other biological fluids

Assay length: 3.5h

Research Area: Tumor immunity;Endocrinology;Reproductive science;Developmental science;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse INHbB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse INHbB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse INHbB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse INHbB in the samples is then determined by comparing the OD of the samples to the standard curve.