ELK016ES-48T, EasyStep Human CP(C-Peptide) ELISA Kit, 48T

Alternative Names: C-Peptide

Species: Human

Assay Type: Sandwich

Sensitivity: 237 pg/mL

Standard: 40 ng/mL

Detection range: 0.63-40 ng/mL

Sample type: Serum, plasma

Assay length: 1.5h

Research Area: Endocrinology;Hormone metabolism;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CP, and the Human CP standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human CP. After TMB substrate solution is added, only those wells that contain Human CP and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CP in the samples is then determined by comparing the OD of the samples to the standard curve.