ELK0162-48T, Rat CA19-9(Carbohydrate Antigen 19-9) ELISA Kit, 48T

Alternative Names: sialylated Lewis(a)Antigen; Cancer Antigen 19-9

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.31 U/mL

Standard: 50 U/mL

Detection range: 0.79-50 U/mL

Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Assay length: 3.5h

Research Area: Tumor immunity;Hepatology;Gastroenterology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CA19-9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CA19-9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CA19-9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CA19-9 in the samples is then determined by comparing the OD of the samples to the standard curve.