ELK015ES-48T, EasyStep Human HBP(Heparin-Binding Protein) ELISA Kit, 48T

Alternative Names: AZU; AZAMP; AZU1; HBP; HUMAZUR; NAZC; CAP37; Cationic Antimicrobial Protein 37; Heparin-Binding Protein; Neutrophil Azurocidin

Species: Human

Assay Type: Sandwich

Sensitivity: 39 pg/mL

Standard: 2500 pg/mL

Detection range: 39-2500 pg/mL

Sample type: Serum

Assay length: 1.5h

Research Area: Enzyme & Kinase;Infection immunity;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HBP, and the Human HBP standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human HBP. After TMB substrate solution is added, only those wells that contain Human HBP and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HBP in the samples is then determined by comparing the OD of the samples to the standard curve.