ELK014ES-48T, EasyStep Human HE4(Epididymal protein 4) ELISA Kit, 48T

Alternative Names: Epididymis Protein 4; dJ461P17.6 ; EDDM4 ; epididymal protein 4 ; Epididymal secretory protein E4 ; Epididymis specific whey acidic protein type four disulfide core ; HE 4 ; Major epididymis specific protein E4 ; Major epididymis-specific protein E4 ; MGC57529 ; Putative protease inhibitor WAP5 ; WAP 5 ; WAP domain containing protein HE4 ; WAP domain containing protein HE4 V4 ; WAP four disulfide core domain 2 ; WAP four disulfide core domain protein 2 ; WAP four-disulfide core domain protein 2 ; WAP5 ; WFDC 2 ; WFDC2

Species: Human

Assay Type: Sandwich

Sensitivity: 160 pg/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: Serum, plasma

Assay length: 1.5h

Research Area: Ovarian cancer

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HE4, and the Human HE4 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human HE4. After TMB substrate solution is added, only those wells that contain Human HE4 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HE4 in the samples is then determined by comparing the OD of the samples to the standard curve.