EasyStep Human PRL(Prolactin) ELISA Kit
Alternative Names: LTH; Luteotropic Hormone
Species: Human
Assay Type: Sandwich
Sensitivity: 248 pg/mL
Standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: Serum
Assay length: 1.5h
Research Area: Endocrinology; Reproductive science; Genetic science; Hormone metabolism
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PRL, and the Human PRL standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human PRL. After TMB substrate solution is added, only those wells that contain Human PRL and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PRL in the samples is then determined by comparing the OD of the samples to the standard curve.
Price | 1.800,00 RON (preturile sunt fara TVA) |
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Description |
Alternative Names: LTH; Luteotropic Hormone Species: Human Assay Type: Sandwich Sensitivity: 248 pg/mL Standard: 20 ng/mL Detection range: 0.32-20 ng/mL Sample type: Serum Assay length: 1.5h Research Area: Endocrinology; Reproductive science; Genetic science; Hormone metabolism Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PRL, and the Human PRL standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human PRL. After TMB substrate solution is added, only those wells that contain Human PRL and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PRL in the samples is then determined by comparing the OD of the samples to the standard curve. |