ELK010ES-48T, EasyStep Human PGII(Pepsinogen II) ELISA Kit, 48T

Alternative Names: PGII

Species: Human

Assay Type: Sandwich

Sensitivity: 7 pg/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: Serum, plasma

Assay length: 1.5h

Research Area: Enzyme & Kinase;Tumor immunity;Gastroenterology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PGII, and the Human PGII standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human PGII. After TMB substrate solution is added, only those wells that contain Human PGII and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PGII in the samples is then determined by comparing the OD of the samples to the standard curve.