ELK005ES-48T, EasyStep Human Cys-C(CystatinC) ELISA Kit, 48T

Alternative Names: CST3; Cystatin 3; ARMD11; Post-Gamma-Globulin

Species: Human

Assay Type: Sandwich

Sensitivity: 25 pg/mL

Standard: 5000 pg/mL

Detection range: 78.1-5000 pg/mL

Sample type: Serum, plasma

Assay length: 1.5h

Research Area: Tags & Cell Markers;Cell Biology;Stem Cells;Cardiovascular

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Cys-C, and the Human Cys-C standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human Cys-C. After TMB substrate solution is added, only those wells that contain Human Cys-C and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Cys-C in the samples is then determined by comparing the OD of the samples to the standard curve.