ELK002ES-48T, EasyStep Human CEA(Carcinoembryonic Antigen) ELISA Kit, 48T

Alternative Names: CD66E; CD66; CEACAM5; Carcinoembryonic Antigen-related Cell Adhesion Molecule 5; Carcinoembryonic Antigen

Species: Human

Assay Type: Sandwich

Sensitivity: 7.5 pg/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: Serum, plasma

Assay length: 1.5h

Research Area: Tumor immunity;Gastroenterology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CEA, and the Human CEA standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human CEA. After TMB substrate solution is added, only those wells that contain Human CEA and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CEA in the samples is then determined by comparing the OD of the samples to the standard curve.