N0459S, dUTP Solution (100 mM) - 25 µmol

N0459S, dUTP Solution (100 mM) - 25 µmol

E1602S, NEB Golden Gate Assembly Kit (BsmBI-v2) - 20 rxns

E1602S, NEB Golden Gate Assembly Kit (BsmBI-v2) - 20 rxns

E8201S, NEBExpress MBP Fusion and Purification System

5.697,72 RON

The NEBExpress® MBP Fusion and Purification System takes advantage of the strong Ptac promoter and the translation initiation signals of maltose binding protein (MBP) to enhance solubility and expression levels of a desired protein in E. coli. The resulting product is an MBP fusion protein, which is then purified by affinity chromatography.

SKU
NEB_E8201S

The NEBExpress® MBP Fusion and Purification System takes advantage of the strong Ptac promoter and the translation initiation signals of maltose binding protein (MBP) to enhance solubility and expression levels of a desired protein in E. coli. The resulting product is an MBP fusion protein, which is then purified by affinity chromatography.

  • Reliable E. coli expression: substantial yields (up to 100 mg/L)
  • Fusion to MBP has been shown to enhance the solubility of proteins expressed in E. coli (1)
  • Two-step purification: amylose elution followed by TEV Protease cleavage and Ni resin isolation results in a highly pure tag-free target protein
  • Gentle elution with maltose; no detergents or harsh denaturants required

 

Mai multe informatii

Mai multe informatii
Price 4.788,00 RON (preturile sunt fara TVA)
Description

The NEBExpress® MBP Fusion and Purification System takes advantage of the strong Ptac promoter and the translation initiation signals of maltose binding protein (MBP) to enhance solubility and expression levels of a desired protein in E. coli. The resulting product is an MBP fusion protein, which is then purified by affinity chromatography.

  • Reliable E. coli expression: substantial yields (up to 100 mg/L)
  • Fusion to MBP has been shown to enhance the solubility of proteins expressed in E. coli (1)
  • Two-step purification: amylose elution followed by TEV Protease cleavage and Ni resin isolation results in a highly pure tag-free target protein
  • Gentle elution with maltose; no detergents or harsh denaturants required