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E5360S, NEBExpress Cell-free E. coli Protein Synthesis System - 10 rxns
1.742,16 RON
The NEBExpress® Cell-free E. coli Protein Synthesis System is an extract-based transcription/translation system derived from E. coli cells that have been engineered for high in vitro synthesis performance. It is designed to synthesize proteins ranging in size from 17-230 kDa in just 2-4 hours under the control of T7 RNA Polymerase.
SKU
NEB_E5360S
The NEBExpress® Cell-free E. coli Protein Synthesis System is an extract-based transcription/translation system derived from E. coli cells that have been engineered for high in vitro synthesis performance. It is designed to synthesize proteins ranging in size from 17-230 kDa in just 2-4 hours under the control of T7 RNA Polymerase.
- Synthesize high yields of protein (typically 0.5 mg/ml)
- Protein can be synthesized and visualized in approximately 2 - 4 hours
- Synthesize various target proteins ranging from 17 to 230 kDa
- Templates can be plasmid DNA, linear DNA or mRNA.
- Minimal protease activity ensures stability of desired target protein
- RNase contamination can be inhibited by the supplied RNase inhibitor, eliminating clean-up steps
- Protein can be isolated with affinity purification or subject to direct functional analysis
- Reactions can be directly loaded onto an SDS-PAGE gel without the need for acetone or TCA precipitation
- Flexible reaction conditions achieve maximum yield; protein synthesis can be sustained for 10 hours at 37°C or up to 24 hours at lower temperatures
- Reactions can be miniaturized or scaled up
- Compatible with the PURExpress Disulfide Bond Enhancer (NEB #E6820) and NEBExpress GamS Nuclease Inhibitor (NEB #P0774)
Advantages and Features
Features
- Quickly generate analytical amounts of protein for further characterization.
- Synthesizes various target proteins ranging in size from 17 to 230 kDa
- Templates can be either plasmid DNA, linear DNA, or mRNA.
- Minimal protease activity ensures stability of desired target protein
- Plasmid template does not require additional clean up steps, RNase contamination can be inhibited by the supplied RNase inhibitor
- Protein can be isolated with affinity purification or subject to direct functional analysis
- Reactions can be directly loaded onto an SDS-PAGE gel without the need for acetone or TCA precipitation
- Flexible reaction conditions to achieve maximum yield: protein synthesis can be sustained for 10 hours at 37°C or up to 24 hours at lower temperatures without supplementation
- Synthesis reactions can be easily miniaturized or scaled up to yield milligram quantities of protein
- Kit contains an optimized T7 RNA Polymerase for maximum performance
Application Features
- High throughput screening and liquid handling
- Mutation studies: effect of point mutations, deletions and insertions, rapidly identify active domains and functional residues
- Epitope mapping and protein folding
- Expression of toxic proteins
Mai multe informatii
| Price | 1.464,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
The NEBExpress® Cell-free E. coli Protein Synthesis System is an extract-based transcription/translation system derived from E. coli cells that have been engineered for high in vitro synthesis performance. It is designed to synthesize proteins ranging in size from 17-230 kDa in just 2-4 hours under the control of T7 RNA Polymerase.
Advantages and Features Features
Application Features
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