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E3030S, Luna Cell Ready One-Step RT-qPCR Kit - 100 rxns
6.754,44 RON
The Luna® Cell Ready One-Step RT-qPCR Kit provides all the necessary components for direct, dye-based RNA detection and quantitation, bypassing the need for RNA extraction and purification. The Luna Cell Ready One-Step RT-qPCR Kit is composed of: 1) the Luna Cell Ready Lysis Module (NEB #E3032S) and 2) the Luna Universal One-Step RT-qPCR Kit (NEB #E3005L).
SKU
NEB_E3030S
The Luna® Cell Ready One-Step RT-qPCR Kit provides all the necessary components for direct, dye-based RNA detection and quantitation, bypassing the need for RNA extraction and purification. The Luna Cell Ready One-Step RT-qPCR Kit is composed of: 1) the Luna Cell Ready Lysis Module (NEB #E3032S) and 2) the Luna Universal One-Step RT-qPCR Kit (NEB #E3005L).
Cell cultures are often analyzed for gene expression or treatment responses as a proxy for a living organism. Traditionally, RNA is extracted and purified from treated cells via column-based or chemical methods. Coordinating the actions of DNase I and the Luna Cell Ready Protease, the Luna Cell Ready Lysis Module offers a simple alternative workflow resulting in effective cell lysis, RNA release, and genomic DNA removal simultaneously in a 15-minute protocol. The Lysis Module includes a unique Luna Cell Ready RNA Protection Reagent that maintains RNA integrity during cell lysis. The lysis capacity spans 10 -100,000 cells in a 50 µl lysis reaction. Up to 2 µl of lysate (equivalent to RNA from 0.2 - 4,000 cells) can be transferred into 20 µl downstream RT-qPCR reactions. Similar to other Luna products, the lysis buffer includes an inert blue tracking dye for visual assistance throughout the workflow.
The Luna Universal One-Step RT-qPCR Kit is compatible with dye-based real-time quantitation of target RNA sequences via the SYBR®/FAM fluorescence channel of most real-time instruments. In the Luna Universal One-Step RT-qPCR Kit, Hot Start Taq DNA polymerase is combined with a novel Luna WarmStart Reverse Transcriptase, allowing dual control of enzyme activity via reversible, aptamer-based inhibition. The Luna Universal One-Step RT-qPCR Kit can detect and quantitate RNA transcripts in the cell lysate prepared directly from the Luna Cell Ready Lysis Module.
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Validated Cell line |
Property |
Species |
Cell number per 50 μl lysate |
|
A549 |
Adherent |
H. sapiens, Lung, carcinoma |
10 - 100,000 |
|
HEK293 |
Adherent |
H. sapiens, Kidney |
10 - 100,000 |
|
HeLa |
Adherent |
H. sapiens, Cervix, adenocarcinoma |
10 - 100,000 |
|
HepG2 |
Adherent |
H. sapiens, Liver, carcinoma |
10 - 1,000 |
|
NCI-H460 |
Adherent |
H. sapiens, Lung, carcinoma |
10 - 100,000 |
|
SK-N-SH |
Adherent |
H. sapiens, Brain, Neuroblastoma |
10 - 10,000 |
|
U2Os |
Adherent |
H. sapiens, Bone, osteosarcoma |
10 - 100,000 |
|
Jurkat |
Suspension |
H. sapiens, T lymphocyte, leukemia |
10 - 100,000 |
|
K-562 |
Suspension |
H. sapiens, Lymphoblast, leukemia |
10 - 100,000 |
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| Price | 5.676,00 RON (preturile sunt fara TVA) | ||||||||||||||||||||||||||||||||||||||||
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| Description |
The Luna® Cell Ready One-Step RT-qPCR Kit provides all the necessary components for direct, dye-based RNA detection and quantitation, bypassing the need for RNA extraction and purification. The Luna Cell Ready One-Step RT-qPCR Kit is composed of: 1) the Luna Cell Ready Lysis Module (NEB #E3032S) and 2) the Luna Universal One-Step RT-qPCR Kit (NEB #E3005L). The Luna Universal One-Step RT-qPCR Kit is compatible with dye-based real-time quantitation of target RNA sequences via the SYBR®/FAM fluorescence channel of most real-time instruments. In the Luna Universal One-Step RT-qPCR Kit, Hot Start Taq DNA polymerase is combined with a novel Luna WarmStart Reverse Transcriptase, allowing dual control of enzyme activity via reversible, aptamer-based inhibition. The Luna Universal One-Step RT-qPCR Kit can detect and quantitate RNA transcripts in the cell lysate prepared directly from the Luna Cell Ready Lysis Module.
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