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E1020-200, PureRec Duplex-Specific Nuclease (DSN) (200 Units)
1.071,00 RON
PureRec Duplex-Specific Nuclease (DSN) is an endonuclease that selectively digests double-stranded DNA (dsDNA) and DNA in DNA-RNA hybrids, exhibiting virtually no activity towards single-stranded DNA (ssDNA) or RNA.
SKU
ZR_E1020-200
PureRec Duplex-Specific Nuclease (DSN) is an endonuclease that selectively digests double-stranded DNA (dsDNA) and DNA in DNA-RNA hybrids, exhibiting virtually no activity towards single-stranded DNA (ssDNA) or RNA.
It can discriminate between perfectly matched and non-matched short DNA duplexes. To catalyze cleavage, DSN needs a minimum of 10 bp dsDNA or 15 bp perfectly matched DNA-RNA duplex.
This enzymatic function is reliant upon the presence of Mg2+ ions, with a recommended concentration of 5 mM for most applications.
PureRec DSN is overexpressed and extensively purified from a non-animal source and is free of non-specific nucleases or RNases.
Application
- Remove gDNA and DNA in DNA:RNA hybrids for protein or RNA preparations.
- Reduce abundant rRNA and globin transcripts to enhance next-generation sequencing (NGS) libraries.
- Achieve precise single-nucleotide polymorphism (SNP) detection with minimal off-target effects.
- Enhance signal amplification for accurate virus detection.
- Improve detection of miRNA and biomarkers.
Technical Specifications
|
Unit Definition |
One Kunitz unit causes an increase in absorbance at 260 nm of 0.001 per minute per ml, at 25°C, pH 7.15, when acting on calf thymus DNA. |
|
Concentration |
1 U/µl |
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Optimal Temperature |
60 °C |
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Reaction Buffer |
10X DSN Digestion Buffer |
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Stop Solution |
2X DSN Stop Solution |
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Storage Condition |
For long-term storage, store at -20 °C. |
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Inactivation |
Add the 2X DSN Stop Solution to the reaction, to a final concentration of 1X, and incubate at the reaction temperature for 5 minutes. |
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| Price | 900,00 RON (preturile sunt fara TVA) | ||||||||||||||
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| Description |
PureRec Duplex-Specific Nuclease (DSN) is an endonuclease that selectively digests double-stranded DNA (dsDNA) and DNA in DNA-RNA hybrids, exhibiting virtually no activity towards single-stranded DNA (ssDNA) or RNA. It can discriminate between perfectly matched and non-matched short DNA duplexes. To catalyze cleavage, DSN needs a minimum of 10 bp dsDNA or 15 bp perfectly matched DNA-RNA duplex. This enzymatic function is reliant upon the presence of Mg2+ ions, with a recommended concentration of 5 mM for most applications. PureRec DSN is overexpressed and extensively purified from a non-animal source and is free of non-specific nucleases or RNases. Application
Technical Specifications
|