DNase I is an endonuclease that nonspecifically cleaves single- and double-stranded DNA. It requires divalent metal cations to be active.
TECHNICAL SPECIFICATIONS
Unit Definition
One Kunitz causes an increase in absorbance at 260 nm of 0.001 per minute per ml, at 25°C, pH 7.5, when acting on salmon sperm DNA according to the assay method of Kunitz. One Unit of DNase I is equivalent to 12 Kunitz.
Storage
After reconstitution, place on ice until ready to use or store frozen aliquots (-20°C).
Reconstitution
Add DNase/RNase-free water to the lyophilized DNase I, mix by gentle inversion. Avoid phosphate buffer and calcium chelators.
Inactivation
Heat inactivate at 75 °C for 10 min with 5 mM EDTA.
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Price
1.105,00 RON (preturile sunt fara TVA)
Description
HIGHLIGHTS
Superior activity.
Certified RNase-free and protease-free.
Withstand more than 10 freeze-thaw cycles.
DESCRIPTION
DNase I is an endonuclease that nonspecifically cleaves single- and double-stranded DNA. It requires divalent metal cations to be active.
TECHNICAL SPECIFICATIONS
Unit Definition
One Kunitz causes an increase in absorbance at 260 nm of 0.001 per minute per ml, at 25°C, pH 7.5, when acting on salmon sperm DNA according to the assay method of Kunitz. One Unit of DNase I is equivalent to 12 Kunitz.
Storage
After reconstitution, place on ice until ready to use or store frozen aliquots (-20°C).
Reconstitution
Add DNase/RNase-free water to the lyophilized DNase I, mix by gentle inversion. Avoid phosphate buffer and calcium chelators.
Inactivation
Heat inactivate at 75 °C for 10 min with 5 mM EDTA.
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