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D6430-PS3, Quick-16S Plus NGS Library Prep Kit (V4) (96 preps) with Index Primer Set 3

D6430-PS3, Quick-16S Plus NGS Library Prep Kit (V4) (96 preps) with Index Primer Set 3

D6432, Quick-16S Plus NGS Library Prep Kit (V4) (384 preps)

D6432, Quick-16S Plus NGS Library Prep Kit (V4) (384 preps)

D6430-PS4, Quick-16S Plus NGS Library Prep Kit (V4) (96 preps) with Index Primer Set 4

8.895,25 RON

The Quick-16S Plus NGS Library Prep Kit (V4) is the fastest and simplest NGS library prep targeting the V4 region of the 16S rRNA gene for high-throughput sequencing. The automation-friendly protocol utilizes a premixed amplification plate and a single qPCR/PCR for combined targeted amplification and barcode addition. Pooling by equal volume and a single clean-up of the final library, rather than massive AMPure® bead-based clean-ups, simplifies the library prep process. Additional library quantification analysis such as TapeStation® analysis or gel electrophoresis are not necessary. With these features, the workflow dramatically reduces the hands-on time of library preparation to only 30 minutes.

SKU
ZR_D6430-PS4
Adaugati pentru comparare
HIGHLIGHTS
  • Fast: 8x less hands-on time than conventional 16S library prep protocols.
  • Easy: Premixed Plate minimizes hands-on time and handling errors.
  • Simple Workflow: 100% Automation ready with only a single PCR step and no need for normalization. Just pool by equal volume!
DESCRIPTION

The Quick-16S Plus NGS Library Prep Kit (V4) is the fastest and simplest NGS library prep targeting the V4 region of the 16S rRNA gene for high-throughput sequencing. The automation-friendly protocol utilizes a premixed amplification plate and a single qPCR/PCR for combined targeted amplification and barcode addition. Pooling by equal volume and a single clean-up of the final library, rather than massive AMPure® bead-based clean-ups, simplifies the library prep process. Additional library quantification analysis such as TapeStation® analysis or gel electrophoresis are not necessary. With these features, the workflow dramatically reduces the hands-on time of library preparation to only 30 minutes.

TECHNICAL SPECIFICATIONS
Amplicon Size The final amplicon size after 1-Step PCR (targeted amplification and barcode addition) is ~388 bp.
Equipment Needed (user provided) Microcentrifuge, plate spinner (centrifuge), 96-well real-time quantitative PCR system (SYBR Green compatible recommended), or standard PCR system, and 96-well real-time PCR plates.
Index Sequences 10 bp indexes are listed in the MiSeq Sample Sheet Template provided.
Sample Input Purified microbial DNA (≤100 ng), free of PCR inhibitors.
Sequencing Platform Compatible with all Illumina® sequencing platforms using custom sequencing primers. We recommend the MiSeq® Reagent Kit v2 (300-cycle).
V4 Primer Sequences (adapters not included) 515f (GTGYCAGCMGCCGCGGTAA) and 806r (GGACTACNVGGGTWTCTAAT).

Mai multe informatii

Mai multe informatii
Price 7.475,00 RON (preturile sunt fara TVA)
Description
HIGHLIGHTS
  • Fast: 8x less hands-on time than conventional 16S library prep protocols.
  • Easy: Premixed Plate minimizes hands-on time and handling errors.
  • Simple Workflow: 100% Automation ready with only a single PCR step and no need for normalization. Just pool by equal volume!
DESCRIPTION

The Quick-16S Plus NGS Library Prep Kit (V4) is the fastest and simplest NGS library prep targeting the V4 region of the 16S rRNA gene for high-throughput sequencing. The automation-friendly protocol utilizes a premixed amplification plate and a single qPCR/PCR for combined targeted amplification and barcode addition. Pooling by equal volume and a single clean-up of the final library, rather than massive AMPure® bead-based clean-ups, simplifies the library prep process. Additional library quantification analysis such as TapeStation® analysis or gel electrophoresis are not necessary. With these features, the workflow dramatically reduces the hands-on time of library preparation to only 30 minutes.

TECHNICAL SPECIFICATIONS
Amplicon Size The final amplicon size after 1-Step PCR (targeted amplification and barcode addition) is ~388 bp.
Equipment Needed (user provided) Microcentrifuge, plate spinner (centrifuge), 96-well real-time quantitative PCR system (SYBR Green compatible recommended), or standard PCR system, and 96-well real-time PCR plates.
Index Sequences 10 bp indexes are listed in the MiSeq Sample Sheet Template provided.
Sample Input Purified microbial DNA (≤100 ng), free of PCR inhibitors.
Sequencing Platform Compatible with all Illumina® sequencing platforms using custom sequencing primers. We recommend the MiSeq® Reagent Kit v2 (300-cycle).
V4 Primer Sequences (adapters not included) 515f (GTGYCAGCMGCCGCGGTAA) and 806r (GGACTACNVGGGTWTCTAAT).