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D6421-PS4, Quick-16S Plus NGS Library Prep Kit (V3-V4) with Primer Set 4

D6421-PS4, Quick-16S Plus NGS Library Prep Kit (V3-V4) with Primer Set 4

D6421-PS6, Quick-16S Plus NGS Library Prep Kit (V3-V4) with Primer Set 6

D6421-PS6, Quick-16S Plus NGS Library Prep Kit (V3-V4) with Primer Set 6

D6421-PS5, Quick-16S Plus NGS Library Prep Kit (V3-V4) with Primer Set 5

8.895,25 RON

The Quick-16S Plus NGS Library Prep Kit (V3-V4, UDI) is the fastest and simplest NGS library prep targeting the V3-V4 region of the 16S rRNA gene for high-throughput sequencing. The automation-friendly protocol utilizes a single qPCR/PCR for combined targeted amplification and barcode addition using specially designed primers. After pooling by equal volume, a single clean-up of the final library is performed, rather than massive AMPure® bead-based clean-ups. Additional library quantification analysis such as TapeStation® analysis or gel electrophoresis are not necessary. With these features, the workflow dramatically reduces the hands-on time of library preparation to only 30 minutes.

SKU
ZR_D6421-PS5
Adaugati pentru comparare
HIGHLIGHTS
  • The most streamlined NGS kit with only 30 minutes of hands-on time for 96 samples.
  • 100% automation ready with only a single PCR step and without the need for normalization.
  • Real-time PCR enables absolute microbial copy number quantification.
DESCRIPTION

 

The Quick-16S Plus NGS Library Prep Kit (V3-V4, UDI) is the fastest and simplest NGS library prep targeting the V3-V4 region of the 16S rRNA gene for high-throughput sequencing. The automation-friendly protocol utilizes a single qPCR/PCR for combined targeted amplification and barcode addition using specially designed primers. After pooling by equal volume, a single clean-up of the final library is performed, rather than massive AMPure® bead-based clean-ups. Additional library quantification analysis such as TapeStation® analysis or gel electrophoresis are not necessary. With these features, the workflow dramatically reduces the hands-on time of library preparation to only 30 minutes.

Click here to learn more.

 

TECHNICAL SPECIFICATIONS
16S V3-V4 Primer Sequences (adapters not included) 341f (CCTACGGGDGGCWGCAG, CCTAYGGGGYGCWGCAG, 17 bp) and 806r (GACTACNVGGGTMTCTAATCC, 24 bp). The forward primer 341f is a mixture of the two sequences listed.
Amplicon Size The final amplicon size after 1-Step PCR (targeted amplification and barcode addition) is ~606 bp.
Barcode Sequences Barcode Sequences: 10 bp barcodes, Available for download here.
Index Primers Unique dual index (barcodes) to label individual samples.
Primer Coverage bacteria (84.2%), archaea (75.8%), and eukaryota (0.0%).
Required Equipment Microcentrifuge, plate spinner (centrifuge), 96-well real-time quantitative PCR system (SYBR Green compatible) or standard PCR system, and 96-well real-time PCR plates.
Sample Input Purified microbial DNA ≤100 ng, free of PCR inhibitors.
Sequencing Platform Illumina MiSeq® without the need to add custom sequencing primers. Zymo Research recommends the MiSeq® Reagent Kit v3 (600-cycle). For assistance with sample sheet setup, see Appendix F.

Mai multe informatii

Mai multe informatii
Price 7.475,00 RON (preturile sunt fara TVA)
Description
HIGHLIGHTS
  • The most streamlined NGS kit with only 30 minutes of hands-on time for 96 samples.
  • 100% automation ready with only a single PCR step and without the need for normalization.
  • Real-time PCR enables absolute microbial copy number quantification.
DESCRIPTION

 

The Quick-16S Plus NGS Library Prep Kit (V3-V4, UDI) is the fastest and simplest NGS library prep targeting the V3-V4 region of the 16S rRNA gene for high-throughput sequencing. The automation-friendly protocol utilizes a single qPCR/PCR for combined targeted amplification and barcode addition using specially designed primers. After pooling by equal volume, a single clean-up of the final library is performed, rather than massive AMPure® bead-based clean-ups. Additional library quantification analysis such as TapeStation® analysis or gel electrophoresis are not necessary. With these features, the workflow dramatically reduces the hands-on time of library preparation to only 30 minutes.

Click here to learn more.

 

TECHNICAL SPECIFICATIONS
16S V3-V4 Primer Sequences (adapters not included) 341f (CCTACGGGDGGCWGCAG, CCTAYGGGGYGCWGCAG, 17 bp) and 806r (GACTACNVGGGTMTCTAATCC, 24 bp). The forward primer 341f is a mixture of the two sequences listed.
Amplicon Size The final amplicon size after 1-Step PCR (targeted amplification and barcode addition) is ~606 bp.
Barcode Sequences Barcode Sequences: 10 bp barcodes, Available for download here.
Index Primers Unique dual index (barcodes) to label individual samples.
Primer Coverage bacteria (84.2%), archaea (75.8%), and eukaryota (0.0%).
Required Equipment Microcentrifuge, plate spinner (centrifuge), 96-well real-time quantitative PCR system (SYBR Green compatible) or standard PCR system, and 96-well real-time PCR plates.
Sample Input Purified microbial DNA ≤100 ng, free of PCR inhibitors.
Sequencing Platform Illumina MiSeq® without the need to add custom sequencing primers. Zymo Research recommends the MiSeq® Reagent Kit v3 (600-cycle). For assistance with sample sheet setup, see Appendix F.