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Digestion of yeast and fungal cell walls is necessary for many experimental procedures. Lytic enzymes like Zymolyase, Lyticase, Glucanase or Chitinase are routinely used for digestion of cell walls. Distinguished from conventional lytic enzymes, Zymolyase Ultra is a novel formulation of enzyme that is optimized for efficient yeast cell wall digestion. It contains enzymes that can degrade several different components of yeast cell walls. It is extensively purified using a novel DNA/RNA removal technology that results in extremely low nucleic acid contamination. It is suitable for qPCR and sequencing-based assays where low DNA/RNA contamination is critical.
Application
- Plasmid, DNA and RNA purification: By breaking down the rigid cell walls of yeast and fungi, Zymolyase Ultra facilitates the release of nucleic acids and significantly increases nucleic acid extraction efficiency.
- Protein purification: Zymolyase Ultra enables efficient extraction of proteins from the cell wall, cell membrane, and intracellular compartments. Unlike mechanical lysis, the gentle enzymatic lysis protects sensitive proteins from denaturation caused by heating or physical shearing. It supports downstream analyses such as protein-protein interaction assays and antibody-based assays, Western blotting, mass spectrometry, and proteomic studies.
- Yeast/fungi detection: Increase cell lysis facilitated by Zymolyase Ultra can directly contribute to enhanced sensitivity of yeast/fungi detection, critical for sensitive analysis like PCR, qPCR and NGS.
- Protoplast/Spheroplast preparation: With gentle enzymatic breakdown of yeast cell walls, cellular structures and components are protected from mechanical stress and damage.
- Yeast genetics and cell biology: Lysing yeast cells using Zymolyase Ultra with ultra-low DNA contamination ensures the extraction of clean and uncontaminated genetic material, minimizing the risk of introducing foreign DNA.
Technical Specifications
|
Activity |
β-1,3-glucan laminaripentao-hydrolase |
|
Format |
Lyophilized |
|
Optimum pH |
7.3 - 8.0 |
|
Optimum Temperature |
37°C |
|
Reconstitution |
Add nucleic acid-free and DNase/RNase-free water to the lyophilized Zymolyase Ultra to 1-5 U/µl, mix by gentle inversion. |
|
Solubility |
≤ 5U/µl in water |
|
Storage |
After reconstitution store frozen aliquots (-20°C). |
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| Price | 4.550,00 RON (preturile sunt fara TVA) | ||||||||||||||
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| Description |
Digestion of yeast and fungal cell walls is necessary for many experimental procedures. Lytic enzymes like Zymolyase, Lyticase, Glucanase or Chitinase are routinely used for digestion of cell walls. Distinguished from conventional lytic enzymes, Zymolyase Ultra is a novel formulation of enzyme that is optimized for efficient yeast cell wall digestion. It contains enzymes that can degrade several different components of yeast cell walls. It is extensively purified using a novel DNA/RNA removal technology that results in extremely low nucleic acid contamination. It is suitable for qPCR and sequencing-based assays where low DNA/RNA contamination is critical. Application
Technical Specifications
|