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D5464, Zymo-Seq SPLAT DNA Library Kit (12 preps)
2.456,30 RON
The Zymo-Seq™ SPLAT DNA Library Kit is a versatile solution for DNA library preparation from a wide variety of sample types. By applying the unique, single-stranded library preparation approach of Splinted Ligation Adapter Tagging (SPLAT), DNA libraries can be prepared from genomic DNA, cell-free DNA, and even FFPE-derived DNA with ease.
SKU
ZR_D5464
The Zymo-Seq™ SPLAT DNA Library Kit is a versatile solution for DNA library preparation from a wide variety of sample types. By applying the unique, single-stranded library preparation approach of Splinted Ligation Adapter Tagging (SPLAT), DNA libraries can be prepared from genomic DNA, cell-free DNA, and even FFPE-derived DNA with ease. Additionally, the SPLAT technology allows for direct ligation of adapters onto the native ends of each DNA fragment, simultaneously eliminating the need for end-repair and ensuring preservation of all original nucleotides. This feature allows for accurate and high-fidelity analysis of fragmentation patterns, setting a new standard for fragmentomics analysis.
The Zymo-Seq™ SPLAT DNA Library Kit easily generates library from 10 ng to 500 ng of pre-fragmented DNA input in just two simple steps: (a) ligation of the single-stranded DNA with the unique splinted adapters concurrently and (b) amplification of the libraries via PCR using unique dual indexes. By utilizing a straightforward workflow that is efficient and user-friendly, the Zymo-Seq™ SPLAT DNA Library Kit allows for generation of high-quality libraries ready for DNA sequencing in as little as 3 hours.
Technical Specifications
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Sample Input Type |
Sonicated genomic DNA (average size of 300-600 bp), sonicated FFPE-derived DNA (average size of 300-600 bp), cfDNA, etc. |
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Minimum Input |
10 ng |
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Maximum Input |
500 ng |
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Input Quality |
For optimal results, input DNA should be free of RNA contamination and enzymatic inhibitors with A260/A280 and A260/A230 values of ≥1.8. DNA with lower purity ratios (A260/A280 & A260/A230) should first be treated with the DNA Clean & Concentrator™ (Cat. No. D4013) prior to processing. DNA should be suspended in water, DNA Elution Buffer, TE buffer, or another similar low-salt buffer. |
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Equipment Required |
Sonication device for DNA input >600 bp in average size, thermal cycler(s) with temperature-adjustable lids, microcentrifuge for 0.2 mL PCR tubes, magnetic stand for 0.2 mL PCR tubes (e.g., PCR Strip MagStand, Cat. No. 3DP-1002). |
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Total Processing Time |
~3 hours |
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Hands-On Time |
~1.5 hours |
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Library Storage |
Libraries eluted in DNA Elution Buffer (provided) may be stored at ≤ 4°C overnight or ≤ -20°C for long-term storage. |
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Sequencing Platform Compatibility |
Libraries are compatible with all Illumina® sequencing platforms. Please see Appendix D for sequencing considerations and recommendations. |
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| Price | 2.030,00 RON (preturile sunt fara TVA) | ||||||||||||||||||
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| Description |
The Zymo-Seq™ SPLAT DNA Library Kit is a versatile solution for DNA library preparation from a wide variety of sample types. By applying the unique, single-stranded library preparation approach of Splinted Ligation Adapter Tagging (SPLAT), DNA libraries can be prepared from genomic DNA, cell-free DNA, and even FFPE-derived DNA with ease. Additionally, the SPLAT technology allows for direct ligation of adapters onto the native ends of each DNA fragment, simultaneously eliminating the need for end-repair and ensuring preservation of all original nucleotides. This feature allows for accurate and high-fidelity analysis of fragmentation patterns, setting a new standard for fragmentomics analysis. The Zymo-Seq™ SPLAT DNA Library Kit easily generates library from 10 ng to 500 ng of pre-fragmented DNA input in just two simple steps: (a) ligation of the single-stranded DNA with the unique splinted adapters concurrently and (b) amplification of the libraries via PCR using unique dual indexes. By utilizing a straightforward workflow that is efficient and user-friendly, the Zymo-Seq™ SPLAT DNA Library Kit allows for generation of high-quality libraries ready for DNA sequencing in as little as 3 hours.
Technical Specifications
|