D5458, Zymo-Seq ATAC Library Kit (12 reactions)

D5458, Zymo-Seq ATAC Library Kit (12 reactions)

D5461, Zymo-Seq RRBS Library Kit (48 preps)

D5461, Zymo-Seq RRBS Library Kit (48 preps)

D5460, Zymo-Seq RRBS Library Kit (24 preps)

8.895,25 RON

Zymo-Seq RRBS Library Kit is an all-in-one RRBS library prep kit that generates NGS libraries with a simplified workflow for the classic method Reduced Representation Bisulfite Sequencing (RRBS). This all-in-one RRBS library prep kit allows for DNA methylation profiling at single-nucleotide resolution in CpG-rich regions of the genome. This Zymo-Seq RRBS Library Kit is the ideal RRBS library prep kit to generate libraries that cover a significant percent (≥70% if using human genomic DNA) of all CpG islands, promotors, and gene bodies while largely reduces the sequencing cost, making it ideal for high-throughput screening and comparison experiments.

SKU
ZR_D5460
HIGHLIGHTS

  • Simple workflow: Prepare Reduced Representation Bisulfite Sequencing (RRBS) libraries in as little as 2 hours of hands-on time.
  • Low input: The only RRBS kit that produces NGS libraries from ≥ 10 ng of genomic DNA.
  • Accurate and reproducible: Unbiased methylation calling and reproducible CpG coverage.
DESCRIPTION

Zymo-Seq RRBS Library Kit is an all-in-one RRBS library prep kit that generates NGS libraries with a simplified workflow for the classic method Reduced Representation Bisulfite Sequencing (RRBS). This all-in-one RRBS library prep kit allows for DNA methylation profiling at single-nucleotide resolution in CpG-rich regions of the genome. This Zymo-Seq RRBS Library Kit is the ideal RRBS library prep kit to generate libraries that cover a significant percent (≥70% if using human genomic DNA) of all CpG islands, promotors, and gene bodies while largely reduces the sequencing cost, making it ideal for high-throughput screening and comparison experiments.

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TECHNICAL SPECIFICATIONS

Equipment Required Microcentrifuge, thermocycler with a heated lid
Input 10 – 500 ng of genomic DNA
Sample Sources Purified genomic DNA. The DNA should be free of enzymatic inhibitors and can be suspended in water, TE, or a low salt buffer. DNA with low 260/280 or 260/230 ratios should be purified prior to processing using the Genomic DNA Clean & Concentrator (Cat. No. D4010). This protocol is not recommended for samples from species with low CpG density.
Sequencing Platform Compatible Libraries are compatible with all Illumina sequencing platforms except HiSeq X Series.
Supplemental Info

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Price 7.475,00 RON (preturile sunt fara TVA)
Description
HIGHLIGHTS

  • Simple workflow: Prepare Reduced Representation Bisulfite Sequencing (RRBS) libraries in as little as 2 hours of hands-on time.
  • Low input: The only RRBS kit that produces NGS libraries from ≥ 10 ng of genomic DNA.
  • Accurate and reproducible: Unbiased methylation calling and reproducible CpG coverage.
DESCRIPTION

Zymo-Seq RRBS Library Kit is an all-in-one RRBS library prep kit that generates NGS libraries with a simplified workflow for the classic method Reduced Representation Bisulfite Sequencing (RRBS). This all-in-one RRBS library prep kit allows for DNA methylation profiling at single-nucleotide resolution in CpG-rich regions of the genome. This Zymo-Seq RRBS Library Kit is the ideal RRBS library prep kit to generate libraries that cover a significant percent (≥70% if using human genomic DNA) of all CpG islands, promotors, and gene bodies while largely reduces the sequencing cost, making it ideal for high-throughput screening and comparison experiments.

Learn More

TECHNICAL SPECIFICATIONS

Equipment Required Microcentrifuge, thermocycler with a heated lid
Input 10 – 500 ng of genomic DNA
Sample Sources Purified genomic DNA. The DNA should be free of enzymatic inhibitors and can be suspended in water, TE, or a low salt buffer. DNA with low 260/280 or 260/230 ratios should be purified prior to processing using the Genomic DNA Clean & Concentrator (Cat. No. D4010). This protocol is not recommended for samples from species with low CpG density.
Sequencing Platform Compatible Libraries are compatible with all Illumina sequencing platforms except HiSeq X Series.
Supplemental Info