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D5023, EZ-96 DNA Methylation-Direct™ Kit (Deep-Well) (2 x 96 Rxns)
3.992,45 RON
The EZ-96 DNA Methylation-Direct Kit is a 96-well bisulfite conversion kit that features reliable and complete DNA bisulfite conversion directly from blood, tissue, and cells without the prerequisite for DNA purification. The increased sensitivity of this 96-well bisulfite conversion kit makes it possible to amplify bisulfite-converted DNA from as few as 10 cells or 50 pg DNA. Recovered bisulfite-converted DNA is ideal for PCR amplification for downstream analyses including restriction endonuclease digestion, sequencing, microarrays, etc.
SKU
ZR_D5023
HIGHLIGHTS
- High-throughput 96-well bisulfite conversion of DNA directly from blood, soft tissue, cells, FFPE samples, and LCM samples.
- Compatible with small sample inputs as few as 10 cells or 50 pg DNA.
- DNA recovered by this bisulfite conversion kit is ideal for downstream analyses including PCR, endonuclease digestion, sequencing, microarrays, etc.
DESCRIPTION
TECHNICAL SPECIFICATIONS
| Applications | Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-generation sequencing, PCR amplification, etc. |
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| Conversion | > 99.5% |
| Elution Volume | Shallow well: > 30 µl Deep-well: >15 µl |
| Equipment | Thermocycler with heated lid, swinging-bucket centrifuge with plate carriers. |
| Input | Samples containing between 50 pg to 2 µg of DNA. For optimal results, the amount of input DNA should be from 200 to 500 ng. The number of cells per standard treatment can range from 10-105 cells. |
| Processing Time | 4 hours |
| Recovery | > 80% |
| Sample Source | Blood, tissue, cells, FFPE, LCM-derived samples, purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. |
| Supplemental Info |
| Price | 3.355,00 RON (preturile sunt fara TVA) | ||||||||||||||||||
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| Description |
HIGHLIGHTS
DESCRIPTIONTECHNICAL SPECIFICATIONS
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| Manufacturer | Zymo Research |
