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The ZymoPURE™ 96 Plasmid Miniprep Kit features a high-throughput method for the purification of up to 100 µg of ultra-pure transfection-grade plasmid DNA using a vacuum manifold or centrifuge. The unique vacuum plate design provides zero buffer retention and low elution volumes. In addition, the provided wash plate also significantly reduces plasmid DNA cross-contamination between wells relative to other popular high-throughput plasmid prep kits.
ZymoPURE™ technology uses a modified alkaline lysis method and our patented binding chemistry, which enables the highest DNA binding capacity and rapid loading of the lysate and wash buffer, resulting in the purification of highly concentrated (up to 1 µg/µl) plasmid DNA directly from a 96-well vacuum plate. In addition, the wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins, salt, protein, and RNA. The result is plasmid DNA suitable for transfection, transformation, lentivirus production, adenovirus production, AAV production, CRISPR, genome editing, sequencing, restriction endonuclease digestion, in vitro transcription/translation, PCR and other sensitive downstream applications.
As an added convenience, the ZymoPURE™ 96 Plasmid Miniprep Kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization. ZymoPURE™ Filter Plates are also included for rapid clearing of the lysate and the unique Zymo-Spin P-96 Plate design allows the binding step to be performed using a vacuum or centrifuge.
Looking to get started with high-throughput plasmid purification? The ZymoPURE 96 Plasmid Starter Package includes a 4 x 96 ZymoPURE 96 Plasmid Miniprep Kit and EZ-Vac 96 Vacuum Manifold, making for a complete high-throughput plasmid purification solution.
Technical Specifications
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Applicable For |
Transfection, transformation, lentivirus production, adenovirus production, AAV production, CRISPR, genome editing, in vivo studies, sequencing, restriction endonuclease digestion, in vitro transcription/translation, PCR, and other sensitive applications. |
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Binding Capacity |
100 µg |
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Culture Input |
≤ 5 mL |
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Elution Volume |
125 µL |
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Endotoxin Levels |
≤ 1 EU/µg |
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Equipment |
96-well plate vacuum manifold (recommended) or swinging bucket centrifuge with microplate adapters. The vacuum pump should be a single or double-staged unit capable of producing up to 300 mm Hg at the vacuum manifold. |
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Format |
96-Well Plate |
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Processing Time |
≤ 60 min |
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Purity |
Typical Abs260/280 ≥ 1.8 and Abs260/230 ≥ 2.0 |
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Recommended Sample Volume |
≤ 5.0 mL |
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Size Range |
Up to ~200 kb |
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Yield |
Up to 100 µg per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. coli utilized. |
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| Price | 7.800,00 RON (preturile sunt fara TVA) | ||||||||||||||||||||||||
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| Description |
The ZymoPURE™ 96 Plasmid Miniprep Kit features a high-throughput method for the purification of up to 100 µg of ultra-pure transfection-grade plasmid DNA using a vacuum manifold or centrifuge. The unique vacuum plate design provides zero buffer retention and low elution volumes. In addition, the provided wash plate also significantly reduces plasmid DNA cross-contamination between wells relative to other popular high-throughput plasmid prep kits. ZymoPURE™ technology uses a modified alkaline lysis method and our patented binding chemistry, which enables the highest DNA binding capacity and rapid loading of the lysate and wash buffer, resulting in the purification of highly concentrated (up to 1 µg/µl) plasmid DNA directly from a 96-well vacuum plate. In addition, the wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins, salt, protein, and RNA. The result is plasmid DNA suitable for transfection, transformation, lentivirus production, adenovirus production, AAV production, CRISPR, genome editing, sequencing, restriction endonuclease digestion, in vitro transcription/translation, PCR and other sensitive downstream applications. As an added convenience, the ZymoPURE™ 96 Plasmid Miniprep Kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization. ZymoPURE™ Filter Plates are also included for rapid clearing of the lysate and the unique Zymo-Spin P-96 Plate design allows the binding step to be performed using a vacuum or centrifuge. Looking to get started with high-throughput plasmid purification? The ZymoPURE 96 Plasmid Starter Package includes a 4 x 96 ZymoPURE 96 Plasmid Miniprep Kit and EZ-Vac 96 Vacuum Manifold, making for a complete high-throughput plasmid purification solution. Technical Specifications
|