D5043, EZ-96 DNA Methylation-Gold MagPrep (8x96 rxns.)
D5042, EZ-96 DNA Methylation-Gold MagPrep (4x96 rxns.)
5.444,25 RON
The EZ-96 DNA Methylation-Gold MagPrep integrates DNA denaturation and bisulfite conversion processes into one step followed by a magnetic bead-based clean-up for high-throughput methylation analysis. To streamline the procedure, high temperature is used instead of sodium hydroxide to denature DNA. Desulphonation and clean-up of the converted DNA is performed while bound to the MagBinding Beads. The EZ DNA Methylation-Gold kits have been designed to minimize template degradation, loss of DNA during treatment and clean-up, and to provide complete conversion of unmethylated cytosines. Recovered DNA is ideal for downstream analyses, including PCR amplification, endonuclease digestion, sequencing, microarrays, etc.
Complete, high-throughput bisulfite conversion of GC-rich DNA in less than 3 hours.
A coupled heat denaturation/conversion reaction step streamlines the conversion of non-methylated cytosines to uracil.
High throughput (96-well), automated desulphonation and recovery of bisulfite-treated DNA. Eluted, ultra-pure DNA is ideal for use in subsequent molecular-based analyses.
DESCRIPTION
The EZ-96 DNA Methylation-Gold MagPrep integrates DNA denaturation and bisulfite conversion processes into one step followed by a magnetic bead-based clean-up for high-throughput methylation analysis. To streamline the procedure, high temperature is used instead of sodium hydroxide to denature DNA. Desulphonation and clean-up of the converted DNA is performed while bound to the MagBinding Beads. The EZ DNA Methylation-Gold kits have been designed to minimize template degradation, loss of DNA during treatment and clean-up, and to provide complete conversion of unmethylated cytosines. Recovered DNA is ideal for downstream analyses, including PCR amplification, endonuclease digestion, sequencing, microarrays, etc.
Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-Generation sequencing, PCR amplification, etc.
Conversion
> 99%
Elution Volume
≥ 25 µl
Equipment
Thermocycler with heated lid, heating element for 96-well plate, magnetic stand.
Input
500 pg - 2 µg of DNA.
Processing Time
3 hours
Recovery
> 70%
Sample Source
Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free.
Complete, high-throughput bisulfite conversion of GC-rich DNA in less than 3 hours.
A coupled heat denaturation/conversion reaction step streamlines the conversion of non-methylated cytosines to uracil.
High throughput (96-well), automated desulphonation and recovery of bisulfite-treated DNA. Eluted, ultra-pure DNA is ideal for use in subsequent molecular-based analyses.
DESCRIPTION
The EZ-96 DNA Methylation-Gold MagPrep integrates DNA denaturation and bisulfite conversion processes into one step followed by a magnetic bead-based clean-up for high-throughput methylation analysis. To streamline the procedure, high temperature is used instead of sodium hydroxide to denature DNA. Desulphonation and clean-up of the converted DNA is performed while bound to the MagBinding Beads. The EZ DNA Methylation-Gold kits have been designed to minimize template degradation, loss of DNA during treatment and clean-up, and to provide complete conversion of unmethylated cytosines. Recovered DNA is ideal for downstream analyses, including PCR amplification, endonuclease digestion, sequencing, microarrays, etc.
Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-Generation sequencing, PCR amplification, etc.
Conversion
> 99%
Elution Volume
≥ 25 µl
Equipment
Thermocycler with heated lid, heating element for 96-well plate, magnetic stand.
Input
500 pg - 2 µg of DNA.
Processing Time
3 hours
Recovery
> 70%
Sample Source
Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free.