D5008, EZ-96 DNA Methylation-Gold™ Kit (Deep-Well) (2 x 96 Rxns)
3.427,20 RON
The EZ-96 DNA Methylation-Gold Kit allows high-throughput (96-well spin-plate) conversion of unmethylated cytosines into uracil in less than 3 hours. Our optimized bisulfite chemistry combines heat denaturation and bisulfite conversion of input DNA to reduce incubation time and ensure conversion rates >99%. Recovered bisulfite-converted DNA is ideal for PCR amplification for downstream analyses including endonuclease digestion, sequencing, microarrays, etc.
High-throughput (96-well) bisulfite conversion of DNA in less than 3 hours. A coupled heat denaturation/conversion reaction step streamlines the conversion of non-methylated cytosines into uracil.
Desulphonation and recovery of bisulfite-treated DNA with a 96-well plate.
Recovered DNA is ideal for downstream analyses such as PCR, endonuclease digestion, sequencing, microarrays, etc.
DESCRIPTION
The EZ-96 DNA Methylation-Gold Kit allows high-throughput (96-well spin-plate) conversion of unmethylated cytosines into uracil in less than 3 hours. Our optimized bisulfite chemistry combines heat denaturation and bisulfite conversion of input DNA to reduce incubation time and ensure conversion rates >99%. Recovered bisulfite-converted DNA is ideal for PCR amplification for downstream analyses including endonuclease digestion, sequencing, microarrays, etc.
TECHNICAL SPECIFICATIONS
Applications
Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-generation sequencing, PCR amplification, etc. Catalog # D5004 is recommended for use with Illumina Infinium MethylationEPIC BeadChip array.
Conversion
>99%
Elution Volume
≥ 15 µl for Deep-well ≥ 30 µl for Shallow-well
Equipment
Thermocycler with heated lid, swinging-bucket centrifuge with plate carriers
Input
500 pg - 2 µg of DNA.
Processing Time
3 hours
Recovery
>75%
Sample Source
Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free.
High-throughput (96-well) bisulfite conversion of DNA in less than 3 hours. A coupled heat denaturation/conversion reaction step streamlines the conversion of non-methylated cytosines into uracil.
Desulphonation and recovery of bisulfite-treated DNA with a 96-well plate.
Recovered DNA is ideal for downstream analyses such as PCR, endonuclease digestion, sequencing, microarrays, etc.
DESCRIPTION
The EZ-96 DNA Methylation-Gold Kit allows high-throughput (96-well spin-plate) conversion of unmethylated cytosines into uracil in less than 3 hours. Our optimized bisulfite chemistry combines heat denaturation and bisulfite conversion of input DNA to reduce incubation time and ensure conversion rates >99%. Recovered bisulfite-converted DNA is ideal for PCR amplification for downstream analyses including endonuclease digestion, sequencing, microarrays, etc.
TECHNICAL SPECIFICATIONS
Applications
Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-generation sequencing, PCR amplification, etc. Catalog # D5004 is recommended for use with Illumina Infinium MethylationEPIC BeadChip array.
Conversion
>99%
Elution Volume
≥ 15 µl for Deep-well ≥ 30 µl for Shallow-well
Equipment
Thermocycler with heated lid, swinging-bucket centrifuge with plate carriers
Input
500 pg - 2 µg of DNA.
Processing Time
3 hours
Recovery
>75%
Sample Source
Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free.