D5003, EZ-96 DNA Methylation™ Kit (Shallow-Well) (2 x 96 Rxns)
3.332,00 RON
The EZ-96 DNA Methylation Kits are bisulfite conversion kits that feature a high-throughput (96-well spin-plate), simplified procedure that streamlines bisulfite conversion of DNA. Cytosines undergo a three-step reaction with sodium bisulfite during which the cytosine is converted into uracil. The innovative in-column desulphonation technology eliminates otherwise cumbersome precipitations. The kit is designed to reduce template degradation and minimize DNA loss during treatment and clean-up, while ensuring complete conversion of the DNA. Purified, converted DNA is ideal for downstream analyses including PCR amplification, endonuclease digestion, sequencing, microarrays, etc.
Streamlined, proven procedure for bisulfite conversion of DNA.
Desulphonation and recovery of bisulfite-treated DNA with a 96-well spin-column plate.
Recovered DNA is ideal for downstream analyses including PCR, endonuclease digestion, sequencing, microarrays, etc.
DESCRIPTION
The EZ-96 DNA Methylation Kits are bisulfite conversion kits that feature a high-throughput (96-well spin-plate), simplified procedure that streamlines bisulfite conversion of DNA. Cytosines undergo a three-step reaction with sodium bisulfite during which the cytosine is converted into uracil. The innovative in-column desulphonation technology eliminates otherwise cumbersome precipitations. The kit is designed to reduce template degradation and minimize DNA loss during treatment and clean-up, while ensuring complete conversion of the DNA. Purified, converted DNA is ideal for downstream analyses including PCR amplification, endonuclease digestion, sequencing, microarrays, etc.
Note: Catalog # D5004 is recommended for use with the Illumina Infinium MethylationEPIC BeadChip array.
TECHNICAL SPECIFICATIONS
Applications
Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-generation sequencing, PCR amplification, etc. Catalog # D5004 is recommended for use with Illumina Infinium MethylationEPIC BeadChip array.
Conversion
>99%
Elution Volume
≥ 15 µl for Deep-well ≥ 30 µl for Shallow-well
Equipment
Thermocycler with heated lid, swinging-bucket centrifuge with plate carriers.
Input
500 pg - 2 µg of DNA
Processing Time
12-16 hours
Recovery
>80%
Sample Source
Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free.
Streamlined, proven procedure for bisulfite conversion of DNA.
Desulphonation and recovery of bisulfite-treated DNA with a 96-well spin-column plate.
Recovered DNA is ideal for downstream analyses including PCR, endonuclease digestion, sequencing, microarrays, etc.
DESCRIPTION
The EZ-96 DNA Methylation Kits are bisulfite conversion kits that feature a high-throughput (96-well spin-plate), simplified procedure that streamlines bisulfite conversion of DNA. Cytosines undergo a three-step reaction with sodium bisulfite during which the cytosine is converted into uracil. The innovative in-column desulphonation technology eliminates otherwise cumbersome precipitations. The kit is designed to reduce template degradation and minimize DNA loss during treatment and clean-up, while ensuring complete conversion of the DNA. Purified, converted DNA is ideal for downstream analyses including PCR amplification, endonuclease digestion, sequencing, microarrays, etc.
Note: Catalog # D5004 is recommended for use with the Illumina Infinium MethylationEPIC BeadChip array.
TECHNICAL SPECIFICATIONS
Applications
Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-generation sequencing, PCR amplification, etc. Catalog # D5004 is recommended for use with Illumina Infinium MethylationEPIC BeadChip array.
Conversion
>99%
Elution Volume
≥ 15 µl for Deep-well ≥ 30 µl for Shallow-well
Equipment
Thermocycler with heated lid, swinging-bucket centrifuge with plate carriers.
Input
500 pg - 2 µg of DNA
Processing Time
12-16 hours
Recovery
>80%
Sample Source
Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free.