The Zyppy-96 Plasmid Miniprep is the fastest and simplest high-throughput plasmid DNA kit available for efficient isolation of plasmid DNA from E. coli. This plasmid DNA kit features a modified alkaline lysis system that is pellet-free. This completely bypasses tedious centrifugation, pelleting, and re-suspension steps common to conventional procedures. Instead, the uniquely formulated Deep Blue Lysis Buffer is added directly to the bacterial cultures in a 96-well block. Buffer neutralization and lysate separation steps are assisted with a specially designed Neutralization/Clearing Buffer. Eluted plasmid DNA is high quality, endotoxin-free, and well-suited for use in restriction endonuclease digestion, DNA ligation, PCR, transcription, sequencing, and other sensitive downstream applications including transfection.
Pellet-Free: Direct lysis procedure omits cell-pelleting and resuspension steps.
High Quality: Ready for PCR, sequencing, cloning, and transfection.
Streamlined Protocol: Culture, lyse, and neutralize in the same plate, saving time and reducing plastic waste.
DESCRIPTION
The Zyppy-96 Plasmid Miniprep is the fastest and simplest high-throughput plasmid DNA kit available for efficient isolation of plasmid DNA from E. coli. This plasmid DNA kit features a modified alkaline lysis system that is pellet-free. This completely bypasses tedious centrifugation, pelleting, and re-suspension steps common to conventional procedures. Instead, the uniquely formulated Deep Blue Lysis Buffer is added directly to the bacterial cultures in a 96-well block. Buffer neutralization and lysate separation steps are assisted with a specially designed Neutralization/Clearing Buffer. Eluted plasmid DNA is high quality, endotoxin-free, and well-suited for use in restriction endonuclease digestion, DNA ligation, PCR, transcription, sequencing, and other sensitive downstream applications including transfection.
TECHNICAL SPECIFICATIONS
Applicable For
Ligation, sequencing, restriction endonuclease digestion, in vitro transcription, and other sensitive applications requiring pure DNA.
Elution Volume
≥ 30 µl
Equipment
Centrifuge with micro plate carriers
Processing Time
45 min
Purity
Typical Abs 260/280 ≥ 1.8.
Size Range
Up to 25 kb
Yield
Up to 5 µg per preparation depending on the plasmid copy number, culture growth conditions, and strain of E. coli processed.
Pellet-Free: Direct lysis procedure omits cell-pelleting and resuspension steps.
High Quality: Ready for PCR, sequencing, cloning, and transfection.
Streamlined Protocol: Culture, lyse, and neutralize in the same plate, saving time and reducing plastic waste.
DESCRIPTION
The Zyppy-96 Plasmid Miniprep is the fastest and simplest high-throughput plasmid DNA kit available for efficient isolation of plasmid DNA from E. coli. This plasmid DNA kit features a modified alkaline lysis system that is pellet-free. This completely bypasses tedious centrifugation, pelleting, and re-suspension steps common to conventional procedures. Instead, the uniquely formulated Deep Blue Lysis Buffer is added directly to the bacterial cultures in a 96-well block. Buffer neutralization and lysate separation steps are assisted with a specially designed Neutralization/Clearing Buffer. Eluted plasmid DNA is high quality, endotoxin-free, and well-suited for use in restriction endonuclease digestion, DNA ligation, PCR, transcription, sequencing, and other sensitive downstream applications including transfection.
TECHNICAL SPECIFICATIONS
Applicable For
Ligation, sequencing, restriction endonuclease digestion, in vitro transcription, and other sensitive applications requiring pure DNA.
Elution Volume
≥ 30 µl
Equipment
Centrifuge with micro plate carriers
Processing Time
45 min
Purity
Typical Abs 260/280 ≥ 1.8.
Size Range
Up to 25 kb
Yield
Up to 5 µg per preparation depending on the plasmid copy number, culture growth conditions, and strain of E. coli processed.