The Zyppy Plasmid Miniprep Kit is a plasmid DNA kit that features a pellet-free modified alkaline lysis system that bypasses the normal cell-pelleting and resuspension steps common to classical plasmid preparation procedures. Simply add the uniquely formulated 7X Lysis Buffer directly to your bacterial culture, neutralize, and then purify with Zymo-Spin column technology. Additionally, the patented colored buffers included in the kit allow for easy visualization of complete cell lysis and neutralization. The Zyppy Plasmid Miniprep Kit is the fastest and easiest method available to separate plasmid DNA from E. Coli efficiently. The plasmid DNA is high quality, endotoxin-free, and well suited for use in transfection, bacterial transformation, restriction endonuclease digestion, DNA ligation, PCR, transcription, sequencing, and other sensitive downstream applications.
Fastest: 8 minutes from culture flask to high-quality plasmid DNA.
Pellet-Free: Direct lysis procedure omits cell-pelleting and resuspension steps.
High Quality: Ready for PCR, sequencing, cloning, and transfection.
DESCRIPTION
The Zyppy Plasmid Miniprep Kit is a plasmid DNA kit that features a pellet-free modified alkaline lysis system that bypasses the normal cell-pelleting and resuspension steps common to classical plasmid preparation procedures. Simply add the uniquely formulated 7X Lysis Buffer directly to your bacterial culture, neutralize, and then purify with Zymo-Spin column technology. Additionally, the patented colored buffers included in the kit allow for easy visualization of complete cell lysis and neutralization. The Zyppy Plasmid Miniprep Kit is the fastest and easiest method available to separate plasmid DNA from E. Coli efficiently. The plasmid DNA is high quality, endotoxin-free, and well suited for use in transfection, bacterial transformation, restriction endonuclease digestion, DNA ligation, PCR, transcription, sequencing, and other sensitive downstream applications.
TECHNICAL SPECIFICATIONS
Applicable For
Ligation, sequencing, restriction endonuclease digestion, in vitro transcription, and other sensitive applications requiring pure DNA.
Elution Volume
≥ 30 µl
Equipment
Microcentrifuge and/or a vacuum manifold.
Processing Time
8 min
Purity
Typical Abs260/280 ≥1.8.
Size Range
Up to 25 kb
Yield
Up to 25 µg of plasmid DNA per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. Coli utilized.
Fastest: 8 minutes from culture flask to high-quality plasmid DNA.
Pellet-Free: Direct lysis procedure omits cell-pelleting and resuspension steps.
High Quality: Ready for PCR, sequencing, cloning, and transfection.
DESCRIPTION
The Zyppy Plasmid Miniprep Kit is a plasmid DNA kit that features a pellet-free modified alkaline lysis system that bypasses the normal cell-pelleting and resuspension steps common to classical plasmid preparation procedures. Simply add the uniquely formulated 7X Lysis Buffer directly to your bacterial culture, neutralize, and then purify with Zymo-Spin column technology. Additionally, the patented colored buffers included in the kit allow for easy visualization of complete cell lysis and neutralization. The Zyppy Plasmid Miniprep Kit is the fastest and easiest method available to separate plasmid DNA from E. Coli efficiently. The plasmid DNA is high quality, endotoxin-free, and well suited for use in transfection, bacterial transformation, restriction endonuclease digestion, DNA ligation, PCR, transcription, sequencing, and other sensitive downstream applications.
TECHNICAL SPECIFICATIONS
Applicable For
Ligation, sequencing, restriction endonuclease digestion, in vitro transcription, and other sensitive applications requiring pure DNA.
Elution Volume
≥ 30 µl
Equipment
Microcentrifuge and/or a vacuum manifold.
Processing Time
8 min
Purity
Typical Abs260/280 ≥1.8.
Size Range
Up to 25 kb
Yield
Up to 25 µg of plasmid DNA per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. Coli utilized.